5VNE

Crystal structure of Sec23a/Sec24a/Sec22 complexed with Emp24 sorting motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77
Detector technology	PIXEL
Collection date	2015-07-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	157.757, 96.501, 126.574
Unit cell angles	90.00, 91.63, 90.00

Refinement procedure

Resolution	49.802 - 2.698
R-factor	0.2261
Rwork	0.224
R-free	0.26620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nut
RMSD bond length	0.002
RMSD bond angle	0.546
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.698
Number of reflections	52341
<I/σ(I)>	2.6
Completeness [%]	99.8
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50mM HEPES, PH 7.8 0.5M NaAc, and 12% PEG4K