5VNC
Glycogen synthase in complex with UDP and glucosamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 193.044, 204.698, 205.888 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.980 |
R-factor | 0.19825 |
Rwork | 0.196 |
R-free | 0.24407 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nb0 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.401 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 145.500 |
High resolution limit [Å] | 2.980 |
Number of reflections | 98100 |
<I/σ(I)> | 14.7 |
Completeness [%] | 95.2 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 273 | 0.1 M Bis-Tris pH 6.1, 22 % Peg 300 |