5VLK
Short PCSK9 delta-P' complex with shrunken peptide bearing homo-Arginine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2017-01-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 70.387, 70.387, 156.983 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 2.200 |
| R-factor | 0.19702 |
| Rwork | 0.195 |
| R-free | 0.23703 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nmx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 23637 | |
| <I/σ(I)> | 16.1 | 2.5 |
| Completeness [%] | 100.0 | 97.2 |
| Redundancy | 9.9 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 292 | calcium acetate, PEG 6000 |
