5VKW
Crystal structure of adenylosuccinate lyase ADE13 from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-03-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 85.030, 157.845, 374.554 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.898 - 1.998 |
| R-factor | 0.1552 |
| Rwork | 0.154 |
| R-free | 0.19460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vd6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.033 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2733: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rpim | 0.063 | 0.662 |
| Number of reflections | 84905 | 4200 |
| <I/σ(I)> | 28.3 | 2.25 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.2 |
| CC(1/2) | 0.576 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M calcium chloride, 0.1 M HEPES pH 7.5, 28% (w/v) PEG400 |
