5VKW

Crystal structure of adenylosuccinate lyase ADE13 from Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-03-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	F 2 2 2
Unit cell lengths	85.030, 157.845, 374.554
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.898 - 1.998
R-factor	0.1552
Rwork	0.154
R-free	0.19460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vd6
RMSD bond length	0.011
RMSD bond angle	1.033
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2733: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.030
High resolution limit [Å]	2.000	2.000
Rpim	0.063	0.662
Number of reflections	84905	4200
<I/σ(I)>	28.3	2.25
Completeness [%]	100.0	100
Redundancy	7.3	7.2
CC(1/2)		0.576

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.2 M calcium chloride, 0.1 M HEPES pH 7.5, 28% (w/v) PEG400