5VJ2
Structure of human respiratory syncytial virus non-structural protein 1 (NS1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.977, 100.682, 122.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.270 - 2.220 |
| R-factor | 0.18814 |
| Rwork | 0.186 |
| R-free | 0.23689 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.202 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.270 |
| High resolution limit [Å] | 2.220 |
| Number of reflections | 13278 |
| <I/σ(I)> | 24.12 |
| Completeness [%] | 98.8 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M MES pH 6.1, 1.5 M ammonium sulfate, and 10% dioxane |
