5VIT
Crystal structure of a Pseudomonas malonate decarboxylase hetero-tetramer in complex with malonate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 |
Unit cell lengths | 93.510, 161.450, 102.720 |
Unit cell angles | 90.80, 93.76, 90.11 |
Refinement procedure
Resolution | 49.043 - 2.203 |
R-factor | 0.188 |
Rwork | 0.186 |
R-free | 0.23220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.192 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.072 | 0.457 |
Number of reflections | 294135 | |
<I/σ(I)> | 9.6 | 1.6 |
Completeness [%] | 97.4 | 95.3 |
Redundancy | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% (w/v) PEG3350 and 8% Tacsimate |