5VIH

Crystal structure of GluN1/GluN2A NMDA receptor agonist binding domains with glycine and antagonist, 4-fluorophenyl-ACEPC

Replaces:  5DE4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	54.736, 87.221, 122.277
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.985 - 2.400
R-factor	0.2052
Rwork	0.200
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nf8
RMSD bond length	0.003
RMSD bond angle	0.609
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.980	2.180
High resolution limit [Å]	2.400	2.110
Number of reflections	25693
<I/σ(I)>	8.78	1.95
Completeness [%]	98.1	86.4
Redundancy	3	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Ammonia Acetate, PEG 4000