5VIG
Crystal structure of anti-Zika antibody Z006 bound to Zika virus envelope protein DIII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 385.082, 385.082, 56.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.749 - 3.000 |
| R-factor | 0.2142 |
| Rwork | 0.212 |
| R-free | 0.25690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.844 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.29) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 96.270 | 96.270 | 3.040 |
| High resolution limit [Å] | 2.900 | 9.620 | 2.900 |
| Rmerge | 0.135 | 0.073 | 0.954 |
| Rmeas | 0.149 | 0.081 | 1.054 |
| Rpim | 0.062 | 0.034 | 0.441 |
| Total number of observations | 189075 | 5317 | 25287 |
| Number of reflections | 34940 | ||
| <I/σ(I)> | 7.5 | 16.7 | 1.7 |
| Completeness [%] | 99.0 | 97.5 | 99.6 |
| Redundancy | 5.4 | 5.4 | 5.4 |
| CC(1/2) | 0.990 | 0.984 | 0.592 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | 10% isopropanol, 0.1M sodium citrate tribasic dihydrate pH 5.0, 26% PEG 400 |
