5VIC
Crystal structure of anti-Zika antibody Z004 bound to DENV-1 Envelope protein DIII
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.225, 74.225, 190.758 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.113 - 3.000 |
R-factor | 0.2369 |
Rwork | 0.235 |
R-free | 0.28580 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.633 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHASER (2.7.16) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 74.230 | 74.230 | 2.830 |
High resolution limit [Å] | 2.700 | 8.960 | 2.700 |
Rmerge | 0.235 | 0.058 | 1.767 |
Rmeas | 0.247 | 0.061 | 1.855 |
Rpim | 0.073 | 0.019 | 0.554 |
Number of reflections | 15181 | ||
<I/σ(I)> | 8.7 | ||
Completeness [%] | 98.6 | 97.3 | 99.6 |
Redundancy | 10.9 | 9.5 | 10.8 |
CC(1/2) | 0.993 | 0.998 | 0.577 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 295 | 0.1M sodium acetate trihydrate pH 4.5, 30% w/v PEG 1500 |