5VHC

DHX36 with an N-terminal truncation bound to ADP-BeF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	61.878, 111.516, 62.966
Unit cell angles	90.00, 110.67, 90.00

Refinement procedure

Resolution	40.496 - 2.490
R-factor	0.1962
Rwork	0.194
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SeMet labeled protein
RMSD bond length	0.002
RMSD bond angle	0.517
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.500
High resolution limit [Å]	2.490
Rmerge	0.075
Rpim	0.043
Number of reflections	25679
<I/σ(I)>	16.85
Completeness [%]	91.5
Redundancy	3.5
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294.15	0.2 M potassium sodium tartrate and 20% w/v PEG 3350