5VGP
Fc fragment of human IgG1 antibody, from NIST mAb
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.912, 79.959, 138.352 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.116 |
R-factor | 0.20315 |
Rwork | 0.201 |
R-free | 0.24494 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3d0s |
RMSD bond length | 0.013 |
RMSD bond angle | 1.656 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | REFMAC (5.8.0155) |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.170 |
High resolution limit [Å] | 2.100 | 2.130 |
Number of reflections | 30746 | |
<I/σ(I)> | 7.2 | |
Completeness [%] | 94.4 | 71 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 17% PEG 8K, 40 mM CaCl2 100 mM Na Hepes |