5VFR
Nucleotide-driven Triple-state Remodeling of the AAA-ATPase Channel in the Activated Human 26S Proteasome
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TECNAI ARCTICA |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.9 |
Software | RELION,Gctf,Coot,PHENIX |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |