5VEZ
DNA polymerase beta substrate complex with 8-oxoG:A at the primer terminus and incoming dCTP analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-03 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.469, 79.712, 55.523 |
| Unit cell angles | 90.00, 107.50, 90.00 |
Refinement procedure
| Resolution | 31.844 - 2.039 |
| R-factor | 0.1907 |
| Rwork | 0.187 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.941 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.039 | 2.050 |
| Rmerge | 0.060 | 0.458 |
| Rpim | 0.383 | |
| Number of reflections | 46175 | |
| <I/σ(I)> | 18.5 | 1.88 |
| Completeness [%] | 99.0 | 96.6 |
| Redundancy | 3.8 | 2.1 |
| CC(1/2) | 0.817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350 |
