5VED
PAK4 kinase domain in complex with Staurosporine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97919 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 62.716, 62.716, 177.197 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.020 - 2.301 |
R-factor | 0.2305 |
Rwork | 0.228 |
R-free | 0.27740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fij |
RMSD bond length | 0.002 |
RMSD bond angle | 0.550 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rpim | 0.089 | 0.946 |
Number of reflections | 16575 | 1608 |
<I/σ(I)> | 8 | 1.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 M Tris-HCl (pH 7.5) and 1.5 - 2.0 M Na acetate |