5VEC
Crystal Structure of the R515L missense variant of human PGM1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2016-10-01 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 171.324, 171.324, 99.382 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.679 - 2.200 |
R-factor | 0.192775380156 |
Rwork | 0.190 |
R-free | 0.25066 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5epc |
RMSD bond length | 0.007 |
RMSD bond angle | 0.853 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX (1.11.1_2575) |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.680 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.279 | 2.141 |
Rpim | 0.084 | 0.720 |
Number of reflections | 75286 | 4379 |
<I/σ(I)> | 11.9 | 1.2 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 11.9 | 9.8 |
CC(1/2) | 0.997 | 0.693 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.01 M Cobalt (II) chloride hexahydrate, O.1 M MES monohydrate pH 6.5, Ammonium Sulfate 1.8-1.9M |