5VDC
Crystal structure of the human DPF2 tandem PHD finger domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.2818, 0.9795 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 107.500, 107.500, 53.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.753 - 1.600 |
| R-factor | 0.1431 |
| Rwork | 0.141 |
| R-free | 0.19060 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.285 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 20713 | 3249 |
| <I/σ(I)> | 28.9 | 2.2 |
| Completeness [%] | 99.0 | 98.3 |
| Redundancy | 26.1 | 26.9 |
| CC(1/2) | 1.000 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 2.2 M Ammonium sulfate, 0.15 M Ammonium citrate |
