5VCS

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase with Bound Acceptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-12
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	46.280, 129.160, 175.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.668 - 2.799
R-factor	0.2486
Rwork	0.247
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vcr
RMSD bond length	0.002
RMSD bond angle	0.445
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.668	72.668	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.147	0.046	0.594
Rmeas	0.166	0.053	0.674
Total number of observations	106149
Number of reflections	25515	342	1850
<I/σ(I)>	8.27	24.9	1.98
Completeness [%]	94.6	90.5	94.3
Redundancy	4.16	4.246	3.928
CC(1/2)	0.990	0.997	0.781

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.6 M ammonium sulfate, 100 mM HEPES, pH 7.5