5VCM
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase with bound UDP and Manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-25 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.130, 139.330, 63.210 |
Unit cell angles | 90.00, 111.08, 90.00 |
Refinement procedure
Resolution | 43.044 - 1.599 |
R-factor | 0.1874 |
Rwork | 0.186 |
R-free | 0.21960 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.887 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.044 | 43.044 | 1.640 |
High resolution limit [Å] | 1.600 | 7.150 | 1.600 |
Rmerge | 0.106 | 0.057 | 1.408 |
Rmeas | 0.114 | 0.061 | 1.514 |
Total number of observations | 745960 | ||
Number of reflections | 97795 | 1125 | 7094 |
<I/σ(I)> | 10.97 | 29.08 | 1.41 |
Completeness [%] | 99.7 | 98.8 | 98.3 |
Redundancy | 7.628 | 7.354 | 7.347 |
CC(1/2) | 0.998 | 0.997 | 0.499 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.7 M ammonium sulfate, 100 mM HEPES, pH 7.5, 5 mM UDP, 5 mM manganese chloride |