5VCB
Crystal structure of holo-(acyl-carrier-protein) synthase:holo(acyl-carrier-protein) complex from Escherichia Coli.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 43 |
| Unit cell lengths | 251.424, 251.424, 58.939 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.308 - 4.100 |
| R-factor | 0.2791 |
| Rwork | 0.278 |
| R-free | 0.29680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vbx |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.996 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.308 | |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.247 | |
| Number of reflections | 28625 | 3890 |
| <I/σ(I)> | 5 | 1.1 |
| Completeness [%] | 95.6 | 80.2 |
| Redundancy | 5.8 | 4.2 |
| CC(1/2) | 0.989 | 0.251 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 100 mM Bis-Tris propane, pH 7.0 18-22% PEG 6k or 18-22% PEG 10k |
