5VBG
Crystal Structure of full-length LpoA, Monoclinic form 1, from Haemophilus influenzae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 145 |
Detector technology | CCD |
Collection date | 2010-02-07 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.458, 69.344, 73.577 |
Unit cell angles | 90.00, 110.18, 90.00 |
Refinement procedure
Resolution | 35.977 - 2.800 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.24640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kcn |
RMSD bond length | 0.002 |
RMSD bond angle | 0.447 |
Data reduction software | HKL-2000 (2.39) |
Data scaling software | HKL-2000 (2.39) |
Phasing software | PHASER |
Refinement software | PHENIX (dev-2376) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.870 |
High resolution limit [Å] | 2.800 | 6.900 | 2.800 |
Rmerge | 0.109 | 0.072 | 0.401 |
Rmeas | 0.131 | 0.087 | 0.526 |
Rpim | 0.072 | 0.047 | 0.336 |
Number of reflections | 14455 | ||
<I/σ(I)> | 7.6 | ||
Completeness [%] | 90.1 | 99.2 | 40.7 |
Redundancy | 3.1 | 3.2 | 1.8 |
CC(1/2) | 0.992 | 0.717 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | Drops were comprised of 1 microliter of concentrated protein (33 mg/ml in 150 mM NaCl, 50 mM Tris-HCl, pH 8.0), 1 microliter precipitant (8% w/v polyethylene glycol 4000, sodium acetate trihydrate, pH 4.6), and 0.2 microliter 30% xylitol. The cryoprotectant was 15% glycerol in the precipitant solution. Crystals frozen in liquid nitrogen. |