5VBF
Crystal structure of succinate semialdehyde dehydrogenase from Burkholderia vietnamiensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-22 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.620, 191.760, 219.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.166 - 2.350 |
R-factor | 0.1476 |
Rwork | 0.147 |
R-free | 0.19600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jz4 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.846 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MoRDa |
Refinement software | PHENIX (dev_2689) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.166 | 48.166 | 2.410 |
High resolution limit [Å] | 2.350 | 10.510 | 2.350 |
Rmerge | 0.158 | 0.063 | 0.517 |
Rmeas | 0.172 | 0.069 | 0.564 |
Number of reflections | 184886 | 2102 | 13707 |
<I/σ(I)> | 10.69 | 23.76 | 3.93 |
Completeness [%] | 98.7 | 90.6 | 99.7 |
Redundancy | 6.379 | 6 | 6.335 |
CC(1/2) | 0.991 | 0.995 | 0.869 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | Rigaku Reagents Morpheus Screen G3: 0.020M Sodium Formate, 0.020M Ammonium Acetate, 0.020M Sodium Citrate, 0.020M Sodium Tartrate, 0.020M Sodium Oxamate, 0.1 M Imidazole/MES pH 6.5, 20% glycerol, 10% PEG 4000: BuviA.00020.v.B1.PS02593 at 14 mg/ml |