5VAY
Bcl-2 complex with Beclin 1 T108D BH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-12 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.205, 53.192, 91.413 |
| Unit cell angles | 90.00, 108.39, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.804 |
| R-factor | 0.1857 |
| Rwork | 0.184 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xa0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.979 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 71186 |
| <I/σ(I)> | 14.41 |
| Completeness [%] | 99.0 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M Tris, pH 6.5, 0.2M ammonium acetate, 25% PEG 3350 |
