5VAX

Bcl-2 complex with Beclin 1 BH3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-09
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	66.474, 87.174, 126.631
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.780 - 2.000
R-factor	0.2109
Rwork	0.209
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xa0
RMSD bond length	0.007
RMSD bond angle	0.833
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	50382
<I/σ(I)>	12.88
Completeness [%]	99.7
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	bis-tris chloride, pH 4.5, 0.2M ammonium acetate, 25% PEG 3350