5VAA
Crystal structure of mouse IgG2a Fc T370K mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.220, 86.350, 67.560 |
| Unit cell angles | 90.00, 111.58, 90.00 |
Refinement procedure
| Resolution | 47.640 - 1.550 |
| R-factor | 0.16978 |
| Rwork | 0.169 |
| R-free | 0.19464 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.776 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.640 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.040 | 0.597 |
| Number of reflections | 77901 | 5739 |
| <I/σ(I)> | 15.47 | 2.41 |
| Completeness [%] | 98.3 | 98.6 |
| Redundancy | 3.32 | 3.41 |
| CC(1/2) | 0.999 | 0.784 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 40% (w/v) PEG 200, 0.1 M MES, pH 6.5 |
