5VA0
Glucocorticoid Receptor DNA Binding Domain in complex with AP-1 response element from VCAM-1 Promoter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.442, 96.280, 105.223 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.483 - 2.295 |
R-factor | 0.1999 |
Rwork | 0.197 |
R-free | 0.23140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hn5 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.972 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.360 |
High resolution limit [Å] | 2.280 | 4.910 | 2.280 |
Rmerge | 0.133 | 0.132 | 0.807 |
Rmeas | 0.145 | 0.144 | 0.877 |
Rpim | 0.056 | 0.056 | 0.338 |
Total number of observations | 130117 | ||
Number of reflections | 18797 | ||
<I/σ(I)> | 7.3 | ||
Completeness [%] | 96.7 | 99.6 | 90.1 |
Redundancy | 6.9 | 6.6 | 6.7 |
CC(1/2) | 0.983 | 0.717 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.05 M Na Cacodylate (pH 7), 5 mM MgCl2, 1 mM Spermine, and 2 % t-Butanol |