5V9X
Structure of Mycobacterium smegmatis helicase Lhr bound to ssDNA and AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.462, 88.129, 83.675 |
| Unit cell angles | 90.00, 109.10, 90.00 |
Refinement procedure
| Resolution | 45.755 - 2.797 |
| R-factor | 0.1991 |
| Rwork | 0.195 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.609 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 99.000 |
| High resolution limit [Å] | 2.797 |
| Number of reflections | 25197 |
| <I/σ(I)> | 15.6 |
| Completeness [%] | 99.0 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 21 - 22 % (w/v) PEG3350, 0.2 M NaF, 0.1 M Bis-Tris Propane (pH 7.0 - 7.2) |
