5V8W
Crystal structure of human Integrator IntS9-IntS11 CTD complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.010, 67.780, 98.585 |
Unit cell angles | 90.00, 100.65, 90.00 |
Refinement procedure
Resolution | 24.830 - 2.100 |
R-factor | 0.1686 |
Rwork | 0.167 |
R-free | 0.22030 |
Structure solution method | SIRAS |
RMSD bond length | 0.012 |
RMSD bond angle | 1.299 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.078 | 0.424 |
Number of reflections | 51362 | 5038 |
<I/σ(I)> | 20.5 | 3.5 |
Completeness [%] | 99.6 | 96.8 |
Redundancy | 6.8 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M Bis-Tris pH 6.5, 21-24% PEG 3350 |