5V8W
Crystal structure of human Integrator IntS9-IntS11 CTD complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.010, 67.780, 98.585 |
| Unit cell angles | 90.00, 100.65, 90.00 |
Refinement procedure
| Resolution | 24.830 - 2.100 |
| R-factor | 0.1686 |
| Rwork | 0.167 |
| R-free | 0.22030 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.299 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.078 | 0.424 |
| Number of reflections | 51362 | 5038 |
| <I/σ(I)> | 20.5 | 3.5 |
| Completeness [%] | 99.6 | 96.8 |
| Redundancy | 6.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M Bis-Tris pH 6.5, 21-24% PEG 3350 |
