5V8I
Thermus thermophilus 70S ribosome lacking ribosomal protein uS17
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 210.805, 450.826, 621.306 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.930 - 3.250 |
| R-factor | 0.2283 |
| Rwork | 0.226 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y4o |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.578 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2747) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.930 | 49.930 | 3.310 |
| High resolution limit [Å] | 3.250 | 17.800 | 3.250 |
| Rmerge | 0.467 | 0.047 | 2.554 |
| Rmeas | 0.488 | 0.049 | 2.680 |
| Rpim | 0.139 | 0.013 | 0.794 |
| Total number of observations | 11330231 | 81963 | 504309 |
| Number of reflections | 918824 | ||
| <I/σ(I)> | 4.9 | 40.9 | 1 |
| Completeness [%] | 100.0 | 95.5 | 100 |
| Redundancy | 12.3 | 14.2 | 11.2 |
| CC(1/2) | 0.991 | 0.999 | 0.290 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.6 | 293 | 0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol |
