5V89
Structure of DCN4 PONY domain bound to CUL1 WHB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 118.930, 62.476, 38.580 |
Unit cell angles | 90.00, 105.48, 90.00 |
Refinement procedure
Resolution | 23.917 - 1.550 |
R-factor | 0.1533 |
Rwork | 0.151 |
R-free | 0.19390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3TDU.pdb |
RMSD bond length | 0.009 |
RMSD bond angle | 1.131 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.610 |
High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
Rmerge | 0.072 | 0.040 | 0.432 |
Number of reflections | 36927 | ||
<I/σ(I)> | 7.7 | ||
Completeness [%] | 93.4 | 99.7 | 60.9 |
Redundancy | 3.5 | 3.8 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 25% PEG3350, 0.2M LiSO4, 10mM TCEP, 10mM Bis-Tris Propane |