5V88
Structure of DCN1 bound to NAcM-COV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.392, 98.677, 59.181 |
| Unit cell angles | 90.00, 104.83, 90.00 |
Refinement procedure
| Resolution | 28.515 - 1.601 |
| R-factor | 0.1608 |
| Rwork | 0.159 |
| R-free | 0.18850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | residues 65-250 of Chain A from 3TDU and residues 6-158 from 2LZM |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.324 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.035 | 0.024 | 0.283 |
| Rmeas | 0.047 | 0.033 | 0.386 |
| Rpim | 0.032 | 0.022 | 0.261 |
| Total number of observations | 87116 | ||
| Number of reflections | 48294 | ||
| <I/σ(I)> | 18.5 | ||
| Completeness [%] | 93.9 | 86.5 | 83.7 |
| Redundancy | 1.8 | 1.9 | 1.7 |
| CC(1/2) | 0.997 | 0.817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 6% PEG3350, 0.2M NH4Br |
