5V7C

Crystal structure of LARP1-unique domain DM15 bound 5'TOP RNA sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-01
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	I 4
Unit cell lengths	107.095, 107.095, 29.113
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.864 - 2.590
R-factor	0.2112
Rwork	0.207
R-free	0.23510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c0v
RMSD bond length	0.015
RMSD bond angle	1.186
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2006: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.864
High resolution limit [Å]	2.590	2.590
Number of reflections	5272
<I/σ(I)>	8.8	1.36
Completeness [%]	98.5	87.1
Redundancy	4.3	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	5 mM MgCl2, 50 mM HEPES, pH 7.0, 25 % PEG MME 550