5V6O

Crystal Structure of the highly open channel-stabilized mutant G-2'I + I9'A of GLIC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-08-04
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97857
Spacegroup name	C 1 2 1
Unit cell lengths	178.388, 133.338, 160.312
Unit cell angles	90.00, 101.77, 90.00

Refinement procedure

Resolution	70.443 - 3.121
R-factor	0.2423
Rwork	0.240
R-free	0.27950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ehz
RMSD bond length	0.011
RMSD bond angle	1.549
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell
Low resolution limit [Å]	105.979	3.132
High resolution limit [Å]	3.121	3.121
Rmerge	0.162	1.090
Rmeas	0.186	1.248
Rpim	0.089	0.602
Total number of observations	276516
Number of reflections	65357
<I/σ(I)>	8.7
Completeness [%]	99.9	99.5
Redundancy	4.2	4.3
CC(1/2)	0.995	0.764

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Sodium Acetate 50 mM pH 3.9-4.2 Ammonium Sulfate 200-250 mM PEG 4000 10-12%