5V6M

Crystal Structure of Rabbit Anti-HIV-1 gp120 V3 Fab 10A3 in complex with V3 peptide ConB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-26
Detector	ADSC QUANTUM 210
Wavelength(s)	0.978637
Spacegroup name	P 21 2 21
Unit cell lengths	62.846, 83.870, 90.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.861 - 1.900
R-factor	0.1808
Rwork	0.178
R-free	0.22430
RMSD bond length	0.006
RMSD bond angle	0.855
Data reduction software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.900	2.000
High resolution limit [Å]	1.900	1.900
Number of reflections	38386	6070
<I/σ(I)>	31.1	5.1
Completeness [%]	99.8	98.8
Redundancy	13.3	10.4
CC(1/2)		0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	32% polyethylene glycol 6000, 1 M LiCL, 0.1 M Tris pH 8.0