5V6E
Crystal structure of Myosin VI in complex with GH2 domain of GIPC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 114.979, 164.096, 171.752 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.082 - 3.506 |
R-factor | 0.1918 |
Rwork | 0.186 |
R-free | 0.23990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5v6h |
RMSD bond length | 0.007 |
RMSD bond angle | 0.823 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.560 |
High resolution limit [Å] | 3.500 | 3.500 |
Rpim | 0.074 | 0.493 |
Number of reflections | 20339 | |
<I/σ(I)> | 10.3 | |
Completeness [%] | 97.0 | 97 |
Redundancy | 6.1 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bis-Tris pH 6.0 and 2 M (NH4)2SO4 |