5V68
Crystal structure of cell division protein FtsZ from Mycobacterium tuberculosis bounded via the T9 loop
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0781 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.110, 180.850, 220.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.050 - 3.460 |
R-factor | 0.24636 |
Rwork | 0.243 |
R-free | 0.30860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2q1y |
RMSD bond length | 0.011 |
RMSD bond angle | 1.470 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.050 | 3.550 |
High resolution limit [Å] | 3.460 | 3.460 |
Rmerge | 0.089 | 0.614 |
Rpim | 0.053 | 0.366 |
Number of reflections | 37287 | 2754 |
<I/σ(I)> | 9.3 | 1.8 |
Completeness [%] | 96.4 | 97.3 |
Redundancy | 3.3 | 3.3 |
CC(1/2) | 0.997 | 0.735 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1M sodium citrate pH 5.6, 0.3M ammonium acetate, 15% PEG 4000. Protein concentration 3mg/ml. Incubated with 5mM SB-P17-A20 |