5V3F

Co-crystal structure of the fluorogenic RNA Mango

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	63.199, 63.199, 138.413
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.668 - 1.700
R-factor	0.1965
Rwork	0.196
R-free	0.20320
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.529
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.700	1.650
High resolution limit [Å]	1.620	1.620
Rmerge		0.350
Number of reflections	29469	2550
<I/σ(I)>	26.87	0.35
Completeness [%]	99.1	82.6
Redundancy	11.1	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	50 mM HEPES pH 7.5, 0.25 M KCL, 1.4 Ammonium Acetate, 2.5 mM Barium Chloride