5V2Q

CaV beta2a subunit: CaV1.2 AID-CEN complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2012-09-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.11587
Spacegroup name	P 21 21 21
Unit cell lengths	54.975, 61.920, 129.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.700
R-factor	0.156
Rwork	0.154
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t3s
RMSD bond length	0.021
RMSD bond angle	1.900
Data reduction software	iMOSFLM (7.0.4)
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER (2.5.1)
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.920	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.079	1.249
Number of reflections	43025
<I/σ(I)>	8.7	0.6
Completeness [%]	87.0	47.7
Redundancy	3.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	1.5-1.7 M ammonium sulfate, 5 mM BME, 0.1 M HEPES, pH 7.0