5V2H
RNA octamer containing glycol nucleic acid, SgnT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-19 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.91836 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.029, 31.901, 33.782 |
Unit cell angles | 90.00, 90.46, 90.00 |
Refinement procedure
Resolution | 33.781 - 1.080 |
R-factor | 0.13663673266 |
Rwork | 0.136 |
R-free | 0.15658 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.303 |
Data scaling software | HKL-2000 (v714) |
Phasing software | SHELXD |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.781 | 1.119 |
High resolution limit [Å] | 1.080 | 1.080 |
Rmerge | 0.164 | 0.265 |
Rpim | 0.069 | 0.265 |
Number of reflections | 35479 | 3532 |
<I/σ(I)> | 10.74 | 2.44 |
Completeness [%] | 97.4 | 97.14 |
Redundancy | 6.3 | 2 |
CC(1/2) | 0.969 | 0.822 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.5 mM oligonucleotide, 40 mM sodium chloride, 10 mM magnesium chloride, 10 mM cobalt(III) hexamine chloride, 20 mM sodium cacodylate, 5% 2-methyl-2,4-pentanediol equilibrated against 40% 2-methyl-2-4-pentanediol |