5V2A

Crystal structure of Fab H7.167 in complex with influenza virus hemagglutinin from A/Shanghai/02/2013 (H7N9)

Replaces:  5F45

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-11
Detector	PSI PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	I 21 3
Unit cell lengths	207.276, 207.276, 207.276
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.348 - 4.656
R-factor	0.261
Rwork	0.258
R-free	0.33350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n5j
RMSD bond length	0.016
RMSD bond angle	2.182
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.730
High resolution limit [Å]	4.650	4.650
Rmerge	0.076	0.972
Rpim	0.019	0.283
Number of reflections	8097	393
<I/σ(I)>	46.7	2.15
Completeness [%]	100.0	100
Redundancy	18.4	12.5
CC(1/2)		0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	10% (w/v) PEG 8000, 0.1 M HEPES, 8% (v/v) ethylene glycol