5V1P

DNA polymerase beta substrate complex with 8-oxoG:C at the primer terminus and incoming dCTP analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-09
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	50.676, 79.990, 55.706
Unit cell angles	90.00, 107.50, 90.00

Refinement procedure

Resolution	24.679 - 1.991
R-factor	0.1792
Rwork	0.175
R-free	0.23100
RMSD bond length	0.007
RMSD bond angle	0.930
Data scaling software	HKL-3000
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.991
Number of reflections	51341
<I/σ(I)>	17.4
Completeness [%]	98.1
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	50 mM imidazole, 350 mM sodium acetate, 17% PEG3350