5V1O

DNA polymerase beta product complex with 8-oxoG:A and inserted dCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-28
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	50.574, 79.685, 55.416
Unit cell angles	90.00, 107.76, 90.00

Refinement procedure

Resolution	31.798 - 1.801
R-factor	0.1806
Rwork	0.179
R-free	0.21500
RMSD bond length	0.007
RMSD bond angle	0.894
Data scaling software	HKL-3000
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Number of reflections	71976
<I/σ(I)>	22.8
Completeness [%]	99.6
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	50 mM imidazole, 350 mM sodium acetate, 17% PEG3350