5V1M
Structure of human Usb1 with uridine 5'-monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.512, 53.219, 46.638 |
| Unit cell angles | 90.00, 107.04, 90.00 |
Refinement procedure
| Resolution | 44.590 - 1.470 |
| R-factor | 0.1611 |
| Rwork | 0.159 |
| R-free | 0.19440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h7w |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.860 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.590 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Number of reflections | 65375 | 1621 |
| <I/σ(I)> | 12.8 | 1.2 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 6.7 | 6.7 |
| CC(1/2) | 0.999 | 0.581 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 20% w/v PEG 20,000, 20% v/v glycerol, 100 mM bis-tris base, 50 mM HCl, and 10 mM disodium 5' UMP |
