5V1L
Structure of S-GNA dodecamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9787 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 34.951, 46.239, 82.928 |
Unit cell angles | 90.00, 100.09, 90.00 |
Refinement procedure
Resolution | 27.600 - 1.200 |
R-free | 0.15300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXDE |
Refinement software | SHELXL |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.600 |
High resolution limit [Å] | 1.200 |
Number of reflections | 40705 |
<I/σ(I)> | 32.34 |
Completeness [%] | 99.8 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | Oligonucleotide (0.6 mM), sodium cacodylate (20 mM, pH 6.0), lithium chloride (20 mM), strontium chloride (40 mM), magnesium chloride (10 mM), spermine tetrahydrochloride (6 mM), and 2-methyl-2,4-pentanediol (MPD; 5% (v/v) |