5V1L

Structure of S-GNA dodecamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9787
Spacegroup name	C 1 2 1
Unit cell lengths	34.951, 46.239, 82.928
Unit cell angles	90.00, 100.09, 90.00

Refinement procedure

Resolution	27.600 - 1.200
R-free	0.15300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXDE
Refinement software	SHELXL

Data quality characteristics

	Overall
Low resolution limit [Å]	27.600
High resolution limit [Å]	1.200
Number of reflections	40705
<I/σ(I)>	32.34
Completeness [%]	99.8
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	Oligonucleotide (0.6 mM), sodium cacodylate (20 mM, pH 6.0), lithium chloride (20 mM), strontium chloride (40 mM), magnesium chloride (10 mM), spermine tetrahydrochloride (6 mM), and 2-methyl-2,4-pentanediol (MPD; 5% (v/v)