5V1D
Complex structure of the bovine PERK luminal domain and its substrate peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2016-10-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.750, 161.547, 104.780 |
| Unit cell angles | 90.00, 106.84, 90.00 |
Refinement procedure
| Resolution | 47.890 - 2.799 |
| R-factor | 0.2447 |
| Rwork | 0.240 |
| R-free | 0.29090 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.710 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.890 |
| High resolution limit [Å] | 2.799 |
| Number of reflections | 47641 |
| <I/σ(I)> | 10.8 |
| Completeness [%] | 91.4 |
| Redundancy | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.1M MES, 1% PEG4000 |
