5V1B
Structure of PHD1 in complex with 1,2,4-Triazolo-[1,5-a]pyridine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 66.717, 112.082, 66.223 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.340 - 2.490 |
R-factor | 0.1917 |
Rwork | 0.189 |
R-free | 0.23630 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.302 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.490 |
Rmerge | 0.100 |
Rmeas | 0.110 |
Rpim | 0.045 |
Total number of observations | 34505 |
Number of reflections | 8476 |
<I/σ(I)> | 7.3 |
Completeness [%] | 96.6 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 273 | 28% PEG 3350, Bis-Tris 7 |