5V0Q
Original engineered variant of I-OnuI meganuclease targeting the HIV integrase gene; harbors 49 point mutations relative to wild-type I-OnuI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 108 |
| Detector technology | PIXEL |
| Collection date | 2016-03-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.693, 74.419, 164.861 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.207 - 2.400 |
| R-factor | 0.1901 |
| Rwork | 0.186 |
| R-free | 0.22980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qqy |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.632 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.101 | 0.066 | 0.800 |
| Rmeas | 0.106 | 0.069 | 0.835 |
| Rpim | 0.030 | 0.021 | 0.237 |
| Total number of observations | 243015 | ||
| Number of reflections | 19980 | 1986 | |
| <I/σ(I)> | 7.7 | ||
| Completeness [%] | 99.9 | 100 | 99.6 |
| Redundancy | 12.2 | 11.8 | 11.8 |
| CC(1/2) | 0.999 | 0.893 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 19% (w/v) PEG 2000, 6% (w/v) PEG-MME 8000, 100mM BIS-TRIS pH 8.0 200mM sodium chloride |
