5V0H
RNA duplex with 2-MeImpG analogue bound-one binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2016-01-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 42.852, 42.852, 83.841 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.110 - 1.900 |
| R-factor | 0.22942 |
| Rwork | 0.227 |
| R-free | 0.27244 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hbx |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.686 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.137 | 0.468 |
| Number of reflections | 7273 | 702 |
| <I/σ(I)> | 10.04 | 2.94 |
| Completeness [%] | 97.4 | 95.4 |
| Redundancy | 7.3 | 4.2 |
| CC(1/2) | 0.957 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 1.8305 M Malonic acid, 0.25 M Ammonium citrate tribasic, 0.12 M Succinic acid, 0.3 M DL-Malic acid, 0.4 M Sodium acetate trihydrate, 0.5 M Sodium formate, and 0.16 M Ammonium tartrate dibasic, pH 7.0 |
