5V0D
Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' flap DNA (f2II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 72.976, 72.976, 180.612 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.980 - 2.630 |
R-factor | 0.208 |
Rwork | 0.205 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qea |
RMSD bond length | 0.002 |
RMSD bond angle | 0.509 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.790 |
High resolution limit [Å] | 2.630 | 2.630 |
Rmerge | 0.054 | 0.593 |
Rmeas | 0.060 | 0.661 |
Number of reflections | 27368 | 4490 |
<I/σ(I)> | 16.08 | 2.17 |
Completeness [%] | 98.9 | 99.7 |
Redundancy | 4.73 | 4.72 |
CC(1/2) | 0.999 | 0.820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 100 mM sodium acetate, pH 7.0, 10 mM potassium chloride, 2-4% PEG4000 |