5V0A

Crystal structure of human exonuclease 1 Exo1 (D225A) in complex with 5' recessed-end DNA (rVIII)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	73.739, 73.739, 179.714
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.370 - 2.380
R-factor	0.214
Rwork	0.211
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qea
RMSD bond length	0.009
RMSD bond angle	0.986
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.410
High resolution limit [Å]	2.370	2.370
Rmerge	0.074	0.635
Rmeas	0.078	0.685
Number of reflections	20768	998
<I/σ(I)>	33.45	2.71
Completeness [%]	99.6	99.2
Redundancy	9.8	7
CC(1/2)	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	100 mM sodium acetate, pH 7.0, 10 mM potassium chloride, 2-4% PEG4000