5V08
Crystal structure of human exonuclease 1 Exo1 (D173A) in complex with 5' recessed-end DNA (rVI)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.259, 74.259, 179.385 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.455 - 2.812 |
R-factor | 0.2186 |
Rwork | 0.215 |
R-free | 0.26230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qea |
RMSD bond length | 0.003 |
RMSD bond angle | 0.489 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1839) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.860 |
High resolution limit [Å] | 2.810 | 2.810 |
Rmerge | 0.077 | 0.684 |
Rmeas | 0.084 | 0.735 |
Number of reflections | 12918 | 640 |
<I/σ(I)> | 25.47 | 2.31 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.4 | 7.5 |
CC(1/2) | 0.888 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 100 mM sodium acetate, pH 7.0, 10 mM potassium chloride, 2-4% PEG4000 |